λ DNA-HindIII Digest _ New England Biolabs

Publish in

Documents

117 views

Please download to get full document.

View again

of 3
All materials on our website are shared by users. If you have any questions about copyright issues, please report us to resolve them. We are always happy to assist you.
Share
Description
λ DNA-HindIII Digest | New England Biolabs https://www.neb.com/products/n3012-dna-hindiii-digest Home › Products › λ DNA-HindIII Digest λ DNA-HindIII Digest Catalog # N3012S N3012L Size 0.3 ml 1.5 ml Concentration 500 μg/ml 500 μg/ml Gel Lanes 150 750 Price $62.00 $248.00 Qty 1 1 Categories: DNA Markers & Ladders Products Applications: DNA Analysis Product Information FAQs Protocols Related Products Description Properties and Usage Description The HindIII digest of lambda DNA (cI857in
Tags
Transcript
  λ DNA-HindIII Digest ProductInformationFAQsProtocolsRelatedProducts DescriptionProperties and Usage Catalog #SizeConcentrationGel LanesPriceQty N3012S0.3 ml500 μg/ml150 $62.00 1 N3012L1.5 ml500 μg/ml750 $248.00 1 Categories: DNA Markers & Ladders Products Applications: DNA Analysis Description The HindIII digest of lambda DNA ( c  I857 ind  1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gelelectrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.Comes supplied with 1 vial of Gel Loading Dye, Blue (6X). Properties and Usage Home › Products › λ DNA-HindIII Digest λ DNA-HindIII Digest | New England Biolabshttps://www.neb.com/products/n3012-dna-hindiii-digest1 of 310/11/2013 3:30 AM  BasesEffective Size Range 125bp to 23,130bp Storage Temperature -20°C Storage Conditions 10 mM Tris-HCl1 mM EDTApH 8.0 @ 25°C Notes For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA maydenature if diluted in dH 2 O and subsequently heated. Temperatures >60°C may cause denaturation.1.The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.2. 1X Gel Loading Dye, Blue:  2.5% Ficoll-40011 mM EDTA3.3 mM Tris-HCL(pH 8.0@25°C)0.017% SDS0.015% bromophenol blue3. References Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A.Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.1.Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.2. Supporting Documents Material Safety Datasheets The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file namingstructure is used to name these document files: [Product Name]MSDS. For international versions please contact us atinfo@neb.com.λ DNA-HindIII Digest MSDS Datacards The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and qualitycontrols. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[LotNumber]. For those product lots not listed below, please contact NEB atinfo@neb.comor fill out theTechnical Support Formfor appropriate document.N3012SDatasheet-Lot1711204N3012SDatasheet-Lot1711209N3012Datasheet-Lot1711204N3012Datasheet-Lot1711209N3012Datasheet-Lot1731209 FragmentMassbp 147723,13021949,41631356,5574904,3615482,3226422,02771256483125 λ DNA-HindIII Digest | New England Biolabshttps://www.neb.com/products/n3012-dna-hindiii-digest2 of 310/11/2013 3:30 AM  λ DNA-HindIII Digest | New England Biolabshttps://www.neb.com/products/n3012-dna-hindiii-digest3 of 310/11/2013 3:30 AM
Related Search
We Need Your Support
Thank you for visiting our website and your interest in our free products and services. We are nonprofit website to share and download documents. To the running of this website, we need your help to support us.

Thanks to everyone for your continued support.

No, Thanks